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phflu4  (New England Biolabs)


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    Structured Review

    New England Biolabs phflu4
    ( A ) Transformation competent cells were prepared from wild-type (“WT”, n = 4) and ΔHindII/III (n = 4) and then electroporated with either pSU20 or <t>pHflu4</t> plasmid DNA. ΔHindII/III produced more colony forming units (CFU) per microgram of transforming DNA (*: p = 0.12, **: p = 0.04). Error bars represent standard error from the mean. ( B ) Restriction digest of plasmids isolated from ΔHindII/III and WT transformants (+: original plasmid used for transformation, L: NEB 1kb plus ladder)
    Phflu4, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phflu4/product/New England Biolabs
    Average 93 stars, based on 25 article reviews
    phflu4 - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Deletion of HindIIR and HindIIIR improves DNA transfer via electroporation to Haemophilus influenzae Rd"

    Article Title: Deletion of HindIIR and HindIIIR improves DNA transfer via electroporation to Haemophilus influenzae Rd

    Journal: bioRxiv

    doi: 10.1101/2024.07.09.602704

    ( A ) Transformation competent cells were prepared from wild-type (“WT”, n = 4) and ΔHindII/III (n = 4) and then electroporated with either pSU20 or pHflu4 plasmid DNA. ΔHindII/III produced more colony forming units (CFU) per microgram of transforming DNA (*: p = 0.12, **: p = 0.04). Error bars represent standard error from the mean. ( B ) Restriction digest of plasmids isolated from ΔHindII/III and WT transformants (+: original plasmid used for transformation, L: NEB 1kb plus ladder)
    Figure Legend Snippet: ( A ) Transformation competent cells were prepared from wild-type (“WT”, n = 4) and ΔHindII/III (n = 4) and then electroporated with either pSU20 or pHflu4 plasmid DNA. ΔHindII/III produced more colony forming units (CFU) per microgram of transforming DNA (*: p = 0.12, **: p = 0.04). Error bars represent standard error from the mean. ( B ) Restriction digest of plasmids isolated from ΔHindII/III and WT transformants (+: original plasmid used for transformation, L: NEB 1kb plus ladder)

    Techniques Used: Transformation Assay, Plasmid Preparation, Produced, Isolation

    ( A ) Red fluorescence provided by pHflu4 is visible in ambient light, while green provided by pHflu6 and pHflu7 is better observed under UV light. H. influenzae ΔHindII/III transformants were serially diluted and spot-plated on sBHI supplemented with 5 µg/ml chloramphenicol and grown for 2 days at 37 °C. The image below was taken under UV light. ( B ) H. influenzae ΔHindII/III carrying pHflu1, pHflu4, pHflu6, and pHflu7 as observed by confocal fluorescence microscopy. Red channel excitation/emission wavelength: 594/613 nm; red channel: 488/543 nm
    Figure Legend Snippet: ( A ) Red fluorescence provided by pHflu4 is visible in ambient light, while green provided by pHflu6 and pHflu7 is better observed under UV light. H. influenzae ΔHindII/III transformants were serially diluted and spot-plated on sBHI supplemented with 5 µg/ml chloramphenicol and grown for 2 days at 37 °C. The image below was taken under UV light. ( B ) H. influenzae ΔHindII/III carrying pHflu1, pHflu4, pHflu6, and pHflu7 as observed by confocal fluorescence microscopy. Red channel excitation/emission wavelength: 594/613 nm; red channel: 488/543 nm

    Techniques Used: Fluorescence, Microscopy



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    phflu4  (New England Biolabs)
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    New England Biolabs phflu4
    ( A ) Transformation competent cells were prepared from wild-type (“WT”, n = 4) and ΔHindII/III (n = 4) and then electroporated with either pSU20 or <t>pHflu4</t> plasmid DNA. ΔHindII/III produced more colony forming units (CFU) per microgram of transforming DNA (*: p = 0.12, **: p = 0.04). Error bars represent standard error from the mean. ( B ) Restriction digest of plasmids isolated from ΔHindII/III and WT transformants (+: original plasmid used for transformation, L: NEB 1kb plus ladder)
    Phflu4, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phflu4/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    phflu4 - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Transformation competent cells were prepared from wild-type (“WT”, n = 4) and ΔHindII/III (n = 4) and then electroporated with either pSU20 or pHflu4 plasmid DNA. ΔHindII/III produced more colony forming units (CFU) per microgram of transforming DNA (*: p = 0.12, **: p = 0.04). Error bars represent standard error from the mean. ( B ) Restriction digest of plasmids isolated from ΔHindII/III and WT transformants (+: original plasmid used for transformation, L: NEB 1kb plus ladder)

    Journal: bioRxiv

    Article Title: Deletion of HindIIR and HindIIIR improves DNA transfer via electroporation to Haemophilus influenzae Rd

    doi: 10.1101/2024.07.09.602704

    Figure Lengend Snippet: ( A ) Transformation competent cells were prepared from wild-type (“WT”, n = 4) and ΔHindII/III (n = 4) and then electroporated with either pSU20 or pHflu4 plasmid DNA. ΔHindII/III produced more colony forming units (CFU) per microgram of transforming DNA (*: p = 0.12, **: p = 0.04). Error bars represent standard error from the mean. ( B ) Restriction digest of plasmids isolated from ΔHindII/III and WT transformants (+: original plasmid used for transformation, L: NEB 1kb plus ladder)

    Article Snippet: Isolated pSU20 and pHflu4 were digested using ApoI-HF (New England Biolabs #R3566) and EcoRI-HF (New England Biolabs # R3101), respectively.

    Techniques: Transformation Assay, Plasmid Preparation, Produced, Isolation

    ( A ) Red fluorescence provided by pHflu4 is visible in ambient light, while green provided by pHflu6 and pHflu7 is better observed under UV light. H. influenzae ΔHindII/III transformants were serially diluted and spot-plated on sBHI supplemented with 5 µg/ml chloramphenicol and grown for 2 days at 37 °C. The image below was taken under UV light. ( B ) H. influenzae ΔHindII/III carrying pHflu1, pHflu4, pHflu6, and pHflu7 as observed by confocal fluorescence microscopy. Red channel excitation/emission wavelength: 594/613 nm; red channel: 488/543 nm

    Journal: bioRxiv

    Article Title: Deletion of HindIIR and HindIIIR improves DNA transfer via electroporation to Haemophilus influenzae Rd

    doi: 10.1101/2024.07.09.602704

    Figure Lengend Snippet: ( A ) Red fluorescence provided by pHflu4 is visible in ambient light, while green provided by pHflu6 and pHflu7 is better observed under UV light. H. influenzae ΔHindII/III transformants were serially diluted and spot-plated on sBHI supplemented with 5 µg/ml chloramphenicol and grown for 2 days at 37 °C. The image below was taken under UV light. ( B ) H. influenzae ΔHindII/III carrying pHflu1, pHflu4, pHflu6, and pHflu7 as observed by confocal fluorescence microscopy. Red channel excitation/emission wavelength: 594/613 nm; red channel: 488/543 nm

    Article Snippet: Isolated pSU20 and pHflu4 were digested using ApoI-HF (New England Biolabs #R3566) and EcoRI-HF (New England Biolabs # R3101), respectively.

    Techniques: Fluorescence, Microscopy